Estimating AAV titer application note
Read this tech note to learn how mass photometry can estimate titers of empty, full and partially filled AAV populations from AAV samples. The note also shows how the AAV titer estimation function in the data acquisition software can be easily activated during routine mass photometry measurements of AAV samples.
Here you can find data that showcases the reproducibility of mass photometry for AAV titer estimation, as well as comparisons with other techniques like ELISA and dPCR.
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Why use mass photometry for AAV titer estimation?
Mass photometers are ideal tools for analysis of AAV samples and AAV titer estimation as they:
- Require very little sample volume and minimal sample preparation
- Are benchtop instruments that require minimal operator training
- Measurements are quick, results in <5 minutes
These strengths make mass photometry an efficient and easy technique to implement in environments that require repeated AAV capsid titer measurement, such as process development
AAV capsid titer is a key analytical attribute of AAV samples
The total concentration of AAV capsid in a sample, or capsid titer, is a critical factor in AAV sample quality, influencing aggregation and representing important information for downstream recovery and purification processes. On the other hand, titer of full AAVs – the concentration of AAV capsids that contain the genetic material to be delivered – is a crucial factor determining the potency of a product and vital information for clinical dosing.
