Rapid analysis of full, partial, and empty ratios for AAV development through manufacturing and QC release.
The Refeyn SamuxMP is a mass photometer optimized for adeno-associated virus (AAV) characterization and is an essential analytical tool for laboratories working with AAVs. SamuxMP mass photometry measurements are quick and require very little sample.
The SamuxMP precisely measures the empty/full capsid ratio for AAVs of any serotype as well as the quantities of partially filled and overfilled capsids. It can also detect impurities and aggregation, and give a rough capsid titer estimate as part of the standard data analysis procedure.
Headline?
Quantitative characterization of AAVs of all serotypes
Easy measurement of empty/full ratio of AAV capsids
Rapid analysis requiring minimal sample
User-friendly operation
Empty/full AAV ratio measurement
Serotype-independent analysis
Partially filled and overfilled capsid quantification
Rough estimation of titer
< 5 minutes to result
Little sample needed
Minimal sample preparation
Very low operating costs
User training < 1 day
Software customized for AAV analysis
Benchtop instrument, easy decontamination
The SamuxMP Auto is the automated mass photometer for AAV characterization, autonomously measuring up to 24 samples in as little as 90 minutes. The instrument rapidly measures AAV empty/full ratios, sample purity and particle aggregation for any AAV serotype – with high precision and reproducibility.
Headline?
Rapid characterization of empty/full AAV capsid ratio
Automated technology – 24 measurements in as little as 90 min
Highly reproducible data with minimal sample preparation
Automation unit retrofittable to the SamuxMP
Empty/full AAV capsid ratio
Partially filled and overfilled capsids
Detection of aggregates and impurities
Rough titer estimation
24 measurements in as little as 90 min
High reproducibility
Automation unit retrofittable to the Samux
Benchtop instrument
Minimal sample preparation
Minimal training required
Start where you are today and scale as you grow – both configurations deliver the same trusted mass photometry results.
For researchers who:
For teams who need:
Mass photometry is a bioanalytical technology that measures the mass of individual AAV particles in solution, quickly and using small amounts of sample.
Mass photometry is ideally suited for measurements of empty/full capsid ratios. Filled AAV capsids have greater mass than empty capsids, so they can be readily distinguished from empty capsids on mass photometry histograms (Fig 1).
The SamuxMP mass photometer can measure AAV capsids of any serotype without the need for protocol adjustments.
Figure 2 shows SamuxMP measurements of empty AAV capsids of four different AAV serotypes.
For each sample, the mass photometry measurements with the SamuxMP revealed a single, symmetric peak at the expected mass, confirming that the SamuxMP produces consistent results across serotypes.
The Samux™ Auto offers all the advantages of AAV capsid analysis with mass photometry while adding convenience and reproducibility via automated, highly consistent sample manipulation (Fig. 1). It accurately measures empty/full capsid ratios of AAVs, and detects partially filled and overfilled AAVs in heterogeneous samples.
The Samux™ Auto can autonomously analyze 24 samples in as little as 90 minutes (up to 180 minutes depending on the protocol, yet fits on a benchtop. Its integrated software suite allows the user to define measurement protocols, analyzes the 24 measurements on the fly, and make it easy to plot and export the resulting data in a variety of formats.
The robotics unit can be retrofitted to existing Samux™ instruments.
This software solution expands the use of the SamuxMP and the SamuxMP Auto mass photometers to GMP – regulated environments and enables their use within AAV-based gene therapy manufacturing pipelines.
The software package supports compliance with the FDA 21 CFR 11 (US) and EU GMP Annex 11 regulations. It includes three applications: ManageMP, AcquireMP and EvaluateMPS.
The Refeyn service teams also provide installation and operational qualifications (IQ/OQ), as well as documentation and on-site training.
Yes, the SamuxMP can distinguish empty, partially filled and full AAV particles.
Yes, the SamuxMP has a virus titer estimation function in its analysis software.
Yes, we recommend a calibration at least once a day. We recommend performing a 1-point calibration with Refeyn’s MassFerence P2.
A software package compliant with FDA and EU GMP Annex 11 regulations is available for use with the SamuxMP and SamuxMP Auto. In addition, the Refeyn service teams provide installation and operational qualifications (IQ/OQ) for instruments destined to GMP-regulated environments.
A software package compliant with FDA and EU GMP Annex 11 regulations is available for use with the SamuxMP and SamuxMP Auto. In addition, the Refeyn service teams provide installation and operational qualifications (IQ/OQ) for instruments destined to GMP-regulated environments.
It can measure 24 samples autonomously including calibrants. After 24 samples, the glass sample carrier slide must be exchanged manually.
Yes, it can do dilutions in the 96-well plate. Dilutions are limited to factors that are multiples of 10.
Yes, we recommend running a calibrant as the first and last sample on every 24-well slide. This will ensure the highest mass accuracy and serve as an internal control. We recommend performing a 1-point calibration with empty AAV capsids of known mass.
A software package compliant with FDA and EU GMP Annex 11 regulations is available for use with the SamuxMP and SamuxMP Auto. In addition, the Refeyn service teams provide installation and operational qualifications (IQ/OQ) for instruments destined to GMP-regulated environments.
MassFerence P2 provides accurate, reproducible AAV mass calibration from 670 kDa to 5 MDa for SamuxMP and SamuxMP Auto systems.
MassGlass UC Sample Prep Kits enable reliable AAV and protein analysis on SamuxMP and TwoMP with clean, ready-to-use slides for reproducible results.
MP Alignment Kit streamlines AAV and protein analysis with SamuxMP and TwoMP for precise, reproducible results.
Read this application note and learn how the SamuxMP mass photometer can quantify the empty/full capsid ratio of AAV samples in downstream purification, while also detecting impurities. Discover the advantages of the SamuxMP, in comparison to the gold-standard techniques such as analytical ultracentrifugation (AUC) and cryogenic transmission electron microscopy (cryoTEM), including its low sample and time requirements.
Read this application note and learn how the SamuxMP mass photometer can measure relative abundance of empty, full as well as heterogeneously loaded viral capsids in an AAV sample. Discover how it compares with analytical ultracentrifugation (AUC) in determining the relative abundances of each AAV population. Mass photometry measurements take only minutes, require minimal training and very small sample volumes, which makes the technology optimal for AAV analytics.
Find out how mass photometry can transform your sample characterization. Whether you’re focused on drug development, discovery science, or manufacturing, Refeyn’s innovative technology provides the insights you need to accelerate your breakthroughs.
We offer a range of mass photometers to suit different applications and budgets. Find the perfect instrument for your lab.
Measure the molecular mass of mRNA, antibodies and other proteins with speed, accuracy, and simplicity. Automated version available.
Check sample purity and homogeneity quickly, easily, and accurately – and run cryo-EM, native MS, SPR, or BLI with confidence.
Rapidly quantify empty, partially filled and full AAV capsids for development and manufacturing. Automated version available.
Gain insights into size, purity and stability to quickly characterize samples of larger viral vectors or virus-like particles.
Rapid analysis of full, partial, and empty ratios for AAV development through manufacturing and QC release.
The SamuxMP mass photometer can measure AAV capsids of any serotype without the need for protocol adjustments.
Figure 2 shows SamuxMP measurements of empty AAV capsids of four different AAV serotypes.
For each sample, the mass photometry measurements with the SamuxMP revealed a single, symmetric peak at the expected mass, confirming that the SamuxMP produces consistent results across serotypes.
The Refeyn SamuxMP is a mass photometer optimized for adeno-associated virus (AAV) characterization and is an essential analytical tool for laboratories working with AAVs. SamuxMP mass photometry measurements are quick and require very little sample.
The SamuxMP precisely measures the empty/full capsid ratio for AAVs of any serotype as well as the quantities of partially filled and overfilled capsids. It can also detect impurities and aggregation, and give a rough capsid titer estimate as part of the standard data analysis procedure.
Quantitative characterization of AAVs of all serotypes
Easy measurement of empty/full ratio of AAV capsids
Rapid analysis requiring minimal sample
User-friendly operation
Empty/full AAV ratio measurement
Serotype-independent analysis
Partially filled and overfilled capsid quantification
Rough estimation of titer
< 5 minutes to result
Little sample needed
Minimal sample preparation
Very low operating costs
User training < 1 day
Software customized for AAV analysis
Benchtop instrument, easy decontamination
Mass photometry is a bioanalytical technology that measures the mass of individual AAV particles in solution, quickly and using small amounts of sample.
Mass photometry is ideally suited for measurements of empty/full capsid ratios. Filled AAV capsids have greater mass than empty capsids, so they can be readily distinguished from empty capsids on mass photometry histograms (Fig 1).
This software solution expands the use of the SamuxMP and the SamuxMP Auto mass photometers to GMP – regulated environments and enables their use within AAV-based gene therapy manufacturing.
The software package supports compliance with the FDA 21 CFR 11 (US) and EU GMP Annex 11 regulations. It includes three applications: ManageMP, AcquireMP and EvaluateMPS.
The Refeyn service teams also provide installation and operational qualifications (IQ/OQ), as well as documentation and on-site training.
Yes, the SamuxMP can distinguish empty, partially filled and full AAV particles.
Yes, the SamuxMP has a virus titer estimation function in its analysis software.
Yes, we recommend a calibration at least once a day. We recommend performing a 1-point calibration with Refeyn’s MassFerence P2.
A software package compliant with FDA and EU GMP Annex 11 regulations is available for use with the SamuxMP and SamuxMP Auto. In addition, the Refeyn service teams provide installation and operational qualifications (IQ/OQ) for instruments destined to GMP-regulated environments.
The SamuxMP Auto is the automated mass photometer for AAV characterization, autonomously measuring up to 24 samples in approximately 90 minutes. The instrument rapidly measures AAV empty/full ratios, sample purity and particle aggregation for any AAV serotype – with high precision and reproducibility.
Read this application note and learn how the SamuxMP mass photometer can measure relative abundance of empty, full as well as heterogeneously loaded viral capsids in an AAV sample. Discover how it compares with analytical ultracentrifugation (AUC) in determining the relative abundances of each AAV population. Mass photometry measurements take only minutes, require minimal training and very small sample volumes, which makes the technology optimal for AAV analytics.
Read this application note and learn how the SamuxMP mass photometer can quantify the empty/full capsid ratio of AAV samples in downstream purification, while also detecting impurities. Discover the advantages of the SamuxMP, in comparison to the gold-standard techniques such as analytical ultracentrifugation (AUC) and cryogenic transmission electron microscopy (cryoTEM), including its low sample and time requirements.
Top up your supply of consumables for analyzing AAV samples with the SamuxMP and SamuxMP Auto mass photometers. Refeyn’s consumables are recommended to ensure consistent measurements.
Purchase the sample preparation packs which include sample carrier slides and sample cassettes with 6 or 24 wells. Order MassFerence P2, a calibrant tailored for AAV analytics with the Samux mass photometers. Click below to order mass photometry consumables now.
