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KaritroMP
Characterization of large vectors for
cell & gene therapy and vaccines

The KaritroMP makes it easy to quickly characterize samples of large viral vectors and virus-like particles (VLPs). Leveraging innovative macro mass photometry technology, the KaritroMP’s unique functionality gives it an edge in terms of speed, ease of use and the information it provides.

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Through single-particle analysis, the KaritroMP enables users to visualize the distributions of particles present in their samples in minutes. Measuring two distinct parameters for every particle, it resolves distinct populations more effectively than other techniques.

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Just as Refeyn’s SamuxMP mass photometer returns game-changing AAV analytics in just minutes and the TwoMP mass photometer offers fast and effective RNA analytics, the KaritroMP quickly returns valuable new insights into the purity, stability and composition of large viral vector samples. The Karitro joins the Samux and TwoMP to create a powerful analytical toolbox for cell and gene therapy, and vaccine development – for every modality.    

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  • Characterization of large viral vectors and VLPs  

  • Semi-automated workflow 

  • Fast and easy to use 

Vital insights

  • Purity 

  • Stability  

  • Size distribution analysis  

Convenience 

  • 6 minutes per sample  

  • Minimal sample prep 

  • Cost-efficient operation with ready-to-use consumables 

Seamless integration

  • Little sample needed (5 μL at 1E8 – 1E9 particles/mL) 

  • Benchtop instrument

  • Insights at a glance 

Schematic graph illustrating data from viral vectors with different sizes but the same mass, or similar sizes but different mass

For each particle in a sample, the KaritroMP measures two distinct parameters: Contrast (a qualitative measure related to the particle’s composition and mass) and size. For further details about how macro mass photometry works, see our overview of macro mass photometry technology.  

Resolution of empty/full ratios

Because it measures contrast and mass, the instrument can distinguish populations of particles with the same size but different mass. For example, it can resolve empty vs. full adenovirus capsids and quantify their proportions (Fig. 1).   â€‹

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Comparing data from the KaritroMP with dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) shows that only the Karitro could resolve the empty and full populations. With both NTA and DLS, only one population was apparent.  

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A Karitro scatter plot shows dots at low and medium size and contrast, with histograms showing ~3 peaks for contrast and 2 peaks for size. NTA and DLS data on size show just one peak each, with the DLS curve much broader.

Its speed and excellent contrast resolution make the KaritroMP the only instrument on the market that can quickly and directly measure AdV empty-full ratios.  

 

Figure 1. The KaritroMP resolves empty/full AdV populations that cannot be resolved using NTA or DLS. Full and empty populations were clearly resolved via KaritroMP analysis of a mixture containing empty, fragmented and full AdV capsids.   

To learn more about this instrument,

Ready-to-use consumables for the KaritroMP

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Enjoy greater reproducibility across your measurements with SizeFerence, a calibrant for the KaritroMP. SizeFerence provides reliable calibrations for macro mass photometry measurements of particles in the 40–150 nm range, including adenoviruses, lentiviruses and VLPs. Contact us to learn more and order now. 

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