Developing and producing cell and gene therapies, as well as mRNA vaccines, requires assessing sample critical quality attributes (CQAs). This includes analyzing vector packaging – the proportion of vectors carrying the therapeutic DNA or RNA cargo – as well as measuring titer and sample purity.
Ideally, vector analytics would be run quickly on little sample, so that production and purification processes can be monitored frequently. The ability to gain insights throughout processing facilitates early identification and correction of faults, speeding up optimization. However, most analytical methods in use today are slow and/or sample-hungry, and cannot be relied on to deliver rapid, in-process information.
Mass photometry and related macro mass photometry, on the other hand, are two technologies offering fast, powerful vector analytics capabilities. Returning results in minutes and using little sample, both technologies offer a way to easily track key attributes during vector production and purification. Based on single-particle analysis, mass photometry technologies also offer exquisite sensitivity, enabling the detection and quantification of distinct subpopulations within samples.
Mass photometry is ideal for use during adeno-associated virus (AAV) production. It can measure not just the AAV empty/full ratio, but also quantify partially filled and overfilled capsids, among other sample attributes. With software that enables use in GMP-compliant settings, mass photometry is also suitable for use in viral vector manufacturing.
Macro mass photometry, meanwhile, can be used to assess the purity and stability of vectors such as adenovirus and lentivirus. It can also, for example, analyze adenovirus packaging, and its unique multiparametric readout correlates with lentivirus physical titer.
With each measurement taking just a few minutes, requiring very little sample and minimal training – mass photometry technologies put the power of single-particle vector analytics within easy reach.
Explore our solutions for vector analytics
Optimize your vector analytics with mass photometry, which offers rapid, precise measurements of AAV empty/full ratios, compatibility with GMP environments and more. Accelerate your AAV production and purification workflows efficiently and accurately.
Measure your adenovirus packaging quickly and easily with macro mass photometry – which can resolve empty vs. full adenovirus populations and assess sample purity and stability. See how macro mass photometry can accelerate your adenoviral vector workflows.
What mass photometry users are saying
Mass photometry solutions for vector analytics
A mass photometer optimized for adeno-associated virus (AAV) characterization, development and manufacturing
A tool for AAV characterization, autonomously measuring up to 24 samples in approximately 90 minutes
Further resources
BLOG POST​
Comparing analytical approaches for AAV characterization
Read this blog to learn how mass photometry measures molecular mass by analyzing light scattering from biomolecules in solution. Discover its direct and rapid results with minimal samples, and its applications in studying proteins, nucleic acids and more, all without requiring modifications.​
​Accelerating protein characterization with mass photometry
Macro mass photometry is a revolutionary new technology for analyzing large viral vectors, such as adenoviruses (AdV) and lentiviruses (LVV). Returning results in minutes, it offers fast, simple, qualitative analysis to inform process development and optimization in the development of cell and gene therapies and vaccines.  Here, you can learn the basic principles of macro mass photometry and its applications.
Video​
Using mass photometry for AAV analytics
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In this short video, Refeyn’s Catia Crespo summarizes how mass photometry can be applied to the analysis of AAVs and its main strengths. She also talks about the support Refeyn provides for implementing mass photometry in GMP-regulated environments.​​
