Accurately measure the mass of single biomolecules in solution, in their native state, and without the need for labels.
Mass photometry is a simple, easy-to-use technology that rapidly analyzes biomolecules at the single-particle level in solution. It measures the light scattered by individual particles and uses the signal to count the particles and measure their mass with high accuracy.
Mass photometry measurements provide the mass distribution and relative concentrations of a wide range of biomolecules in a sample, accelerating a variety of studies, including:
• Sample purity and homogeneity
• Protein oligomerization
• Biomolecular interactions
• Macromolecular complex assembly
• In solution, in a variety of buffers and compatible with membrane proteins
• Label-free, without the need to modify samples
• Single molecule counting
• Wide mass range and high dynamic range
• Homogeneity, structural integrity and activity
• Quick, simple, minimal sample amounts
Mass photometry is a new way to analyze biomolecules, built upon the principles of interference reflection microscopy and interferometric scattering microscopy. Mass photometry enables the reliable detection of single molecules and the direct measurement of each molecule’s mass in solution.
With a wide dynamic range, mass photometry can be used to assess:
• Proteins / glycoproteins
• Nucleic Acids
• Adeno-associated viruses (AAVs)
• Antibodies
• VLPs
• Membrane proteins
Figure: The principle of mass photometry. The light scattered by a molecule that has landed on a measurement surface interferes with light reflected by that surface. The interference signal scales linearly with mass.
Mass photometry enables precise quantification of the interactions between individual antibody molecules (Herceptin) with target molecules (HER2) in solution.
Our mass photometry systems support research, quality control, and manufacturing applications.
Measure molecular mass of biomolecules with speed, accuracy, and simplicity. Automated version available.
Combine the ease and efficiency of automation with the sensitivity and speed of mass photometry for rapid, accurate, multi-sample measurement.
Quantitatively characterize adeno-associated viruses (AAVs) of all serotypes. An automated, high-throughput version is also available.
Mass photometry publications
 – Tim Sharpe, Biozentrum, University of Basel
Explore the science of mass photometry and see how it is used to characterize protein purity, interactions, and oligomerization.
Learn the principles behind mass photometry and explore the unique benefits of this technology for biomolecule analysis.
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