Rapid analysis for AAV development
Adeno-associated viruses (AAVs) have become one of the most used vectors to deliver gene therapies. As their use grows, so does the need for accurate, efficient AAV analytics. Mass photometry can monitor sample critical quality attributes (CQAs) throughout upstream and downstream viral vector development and manufacturing workflows.
Mass photometry can characterize AAV samples with accuracy comparable to widely used legacy methods for AAV vector analytics, such as analytical ultracentrifugation (AUC), and transmission electron microscopy (TEM). Mass photometers like the SamuxMP and the SamuxMP Auto provide rapid and comprehensive AAV packaging analysis suitable for research, process development, and GMP manufacturing.
Measurements with single-particle resolution across a broad dynamic range
Intuitive instruments and software take you from sample to result in less than 5 minutes
Software modules enable compliance in GMP- regulated manufacturing environments
Mass photometry capabilities for AAV characterization and analysis
Rapid capsid content analysis
Mass photometry can quickly quantitate empty, full, and partially filled capsids, making it an ideal analytical tool when optimizing and monitoring AAV purification processes
Accurate measurement of genome length
The mass difference between empty and full AAV population corresponds to the mass of the load, making it straightforward to measure the genomic length of AAV cargo
Results comparable to legacy methods
Mass photometry challenges the status quo by providing serotype-agnostic AAV characterization results comparable to AUC, SEC-MALS, CDMS and cryo-TEM
Efficient upstream AAV characterization
Make upstream AAV process decisions faster using mass photometry partnered with a simple, plate-based cleanup method for AAV culture lysates
Estimation of viral titer
Coupled with reliable capsid content analysis, mass photometry can also be used to calculate rough estimations of viral titer – enabling rapid clone screening and analysis
Compliance in GMP-environments
SamuxMP software modules support compliance in regulated manufacturing environments governed by US FDA 21 CFR Part 11 and EU GMP Annex 11 regulations
AAV vector characterization and analysis with the SamuxMP
- SamuxMP
-
A mass photometer optimized for adeno-associated virus (AAV) characterization, the SamuxMP is an essential tool for AAV research, process development and GMP manufacturing.
- Comprehensive AAV capsid analysis including empty, full, partial ratio and genome length
- Suitable for upstream and downstream process development and analysis
- Software modules to enable compliance in regulated manufacturing environments
- SamuxMP Auto
-
The SamuxMP Auto is the automated version of Refeyn’s solution for AAV characterization, autonomously measuring up to 24 samples in approximately 90 minutes.
- Software
-
SamuxMP instrumentation and software are suitable for research,
process development and GMP manufacturing environments.
Used for instrument operation as well as data acquisition and processing
Loads data from AcquireMP and determines the empty/full/partial AAV ratios through an automated workflow. After analysis, data can be exported as a pdf document signed in human-readable form.
Handles system configuration, user creation, and access control, including permissions for AcquireMP and EvaluateMPS
- Consumables
-
Refeyn consumables are quality tested for consistent measurements and mass photometry results
- Calibrants – MassFerence P2 was designed for use with the SamuxMP for AAV analysis
- Slide preparation kits – Includes sample well cassettes and slides
- Alignment / starter kits – Everything you need to get going including slide-cassette alignment tool, tweezers and magnetic slide holders
- Read the tech note: Accurate calibrations for AAV samples with MassFerence P2
A mass photometer optimized for adeno-associated virus (AAV) characterization, the SamuxMP is an essential tool for AAV research, process development and GMP manufacturing.
- Comprehensive AAV capsid analysis including empty, full, partial ratio and genome length
- Suitable for upstream and downstream process development and analysis
- Software modules to enable compliance in regulated manufacturing environments
The SamuxMP Auto is the automated version of Refeyn’s solution for AAV characterization, autonomously measuring up to 24 samples in approximately 90 minutes.
SamuxMP instrumentation and software are suitable for research,
process development and GMP manufacturing environments.
Used for instrument operation as well as data acquisition and processing
Loads data from AcquireMP and determines the empty/full/partial AAV ratios through an automated workflow. After analysis, data can be exported as a pdf document signed in human-readable form.
Handles system configuration, user creation, and access control, including permissions for AcquireMP and EvaluateMPS
Refeyn consumables are quality tested for consistent measurements and mass photometry results
- Calibrants – MassFerence P2 was designed for use with the SamuxMP for AAV analysis
- Slide preparation kits – Includes sample well cassettes and slides
- Alignment / starter kits – Everything you need to get going including slide-cassette alignment tool, tweezers and magnetic slide holders
- Read the tech note: Accurate calibrations for AAV samples with MassFerence P2
What mass photometry users are saying
BLOG POST: Blog: Comparing analytical approaches for AAV characterization
Read this blog post to learn how mass photometry compares to other AAV analytical methods for measurements of empty/full and heterogeneously loaded AAV capsids.
