Mass photometry publication library

Movie S1. 30 s of a representative ratiometric movie of a solution MP measurement of a mixture of wtSpike at a final concentration of 16.7 nM trimer with an ACE2 final concentration of 100 nM of monomer. The field of view is 3.8x10.7 ?m2, and the contrast scale is from -0.01 to +0.01. The raw frames were…
From Roi Asor et al. (2024), Proceedings of the National Academy of Sciences of the United States of America: Oligomerization-driven avidity correlates with SARS-CoV-2 cellular binding and inhibition · view figure

Fig. 3. Assessment of ACTN2 dimer integrity in ACTN2-R327C and ACTN2-R457C variants.A The estimated molecular weights of WT, R327C, and R457C at time zero (0 h) are assessed using mass photometer (Refeyn), revealing that all samples correspond to the dimer peak (~ 200 kDa) (left panel). After 48 h of…
From Maya Noureddine et al. (2026), Nature communications: Comprehensive biophysical and structural profiling of alpha-actinin-2 variants reveals mechanistic diversity in hypertrophic cardiomyopathy · view figure

Figure 4. Mass photometry of purified rAAVs obtained from plasmid-based rAAV production with SamuxMP (Refeyn). n = 3 biological replicates were measured for each condition and peaks were evaluated using Gaussian peak fitting, resulting in percent filled rAAVs (mean listed next to heading). (A) HEK293 triple…
From Mária Tóth et al. (2026), International journal of molecular sciences: Comparative Analysis of rAAV Production from Plasmid-Encoded Versus Chromosomally Integrated rAAV Transgene in HEK293 Cells · view figure

Figure 5. Mass photometry. (A) Mass photometry results for NS2B in LMNG after preincubation in the detergents indicated; (B) sum of multiple profiles in independent samples after OG preincubation; (C) result after OG preincubation using a Refeyn Microfluidics Chip.
From Caleb Ponniah et al. (2026), International journal of molecular sciences: Zika NS2B Protein: In Vitro Formation of Large Multimeric Networks · view figure

Figure 6. MP histograms of WT, 3D1, 3D2, and 6D Nps at 25 nM final concentration. Histograms shown are combined averages of three measurements. Normalization, averaging, and all other calculations performed using Refeyn DiscoverMP software. Estimated mass of each peak is indicated along with the percent of…
From Megan S. Sullivan et al. (2026), Viruses: Mechanism of SARS-CoV-2 Nucleocapsid Protein Phosphorylation-Induced Functional Switch · view figure

Figure 7. MP analysis of WT Np and 6D Np with (+XL) and without (−XL) crosslinking to SARS-CoV-2 5′UTR RNA constructs. Data were collected at 100 nM final concentrations of each component, and histograms shown are combined averages of three measurements. Normalization and averaging performed using Refeyn…
From Megan S. Sullivan et al. (2026), Viruses: Mechanism of SARS-CoV-2 Nucleocapsid Protein Phosphorylation-Induced Functional Switch · view figure

Fig. 6. Resolved Structure of IA107-bound p-IRE1α and the selectivity profile of IA107.a Back-to-back dimer of IA107-bound p-IRE1α (chain A and chain B). b Hydrogen bond interactions between IA107 and p-IRE1α. c Superimposition view of the kinase dimer interaction from the IA107–p-IRE1α AB dimer (wheat,…
From Yang Liu et al. (2025), Nature communications: Harnessing indole scaffolds to identify small-molecule IRE1α inhibitors modulating XBP1 mRNA splicing · view figure

Fig. 2. Substrates used to probe mechanism of NdasCDO, effect of pH, temperature and salt concentration in the reaction.a Criteria for usage of distinct CDP substrates for different experiments: cGG is a reaction control as it cannot be oxidized; catalysis with cFG is slow and a single oxidation is…
From Emmajay Sutherland et al. (2025), Nature Communications: Broad substrate scope C-C oxidation in cyclodipeptides catalysed by a flavin-dependent filament · view figure

Fig. 5. Mutating the Mcm4 Walker B motif stabilises the Mcm ring.a Schematic showing the pulldown assay used to assess ring splitting. Magnetic beads (brown spheres) were used to pulldown MCM2-7 using the HA-tag on Mcm3. During a high salt wash (H), ATP hydrolysis results in splitting of the ring at the…
From Sarah V Faull et al. (2025), Nature communications: MCM2-7 ring closure involves the Mcm5 C-terminus and triggers Mcm4 ATP hydrolysis · view figure

Figure 5. The charge state at the DNA binding site alters oligomerization. A, peptide hydrolysis by Lon (100 nM hexamer concentration) variants with an ATP regeneration mix and 125 μg/ml casein. Assays were done in triplicate with one replicate shown. B, mass photometry analysis of Lon variants (200 nM…
From Justyne L Ogdahl et al. (2024), The Journal of biological chemistry: Allosteric modulation of the Lon protease via ssDNA binding and local charge changes · view figure