The new KaritroMP is a benchtop instrument that characterizes large viral vectors. Ideal for informing process development in preclinical settings and early drug development, it analyzes samples of adenovirus vectors (AdV) and lentivirus vectors (LVV) in just minutes. The instrument offers essential vector analysis capabilities, including the assessment of sample purity and stability.
Using novel macro mass photometry technology, this pioneering tool performs single-particle analysis, enabling users to visualize distributions of viral particle populations within their samples. It can resolve distinct populations of vectors and reveal changes over time – indicating whether a sample is suitable for further processing and helping users save time and resources.
Fast, simple, qualitative AdV and LVV analysis
Differentiation of multiple populations, even those of the same size
Evaluation of relative changes across samples, from crude to purified
Empty/full measurement for AdV
Analysis of purity and stability for AdV and LVV
6 minutes per sample
Little sample needed (5 μL at 1E8 – 1E9 particles/mL)
Minimal sample preparation
Benchtop instrument
Compare production or purification methods
Identify batch-to-batch variations
For each particle in a sample, the KaritroMP measures two distinct parameters: Contrast (a qualitative measure related to the particle’s composition and mass) and size. This measurement supports multiple applications, including:
Measurement of AdV empty/full ratios
Assessing the effectiveness of sample purification processes
Measuring degradation in viral vector samples
Comparing production methods
Identifying batch-to-batch variations
A custom-designed sample carrier will also be available for use with this instrument.
Because it measures contrast and mass, the instrument can distinguish populations of particles with the same size but different mass. For example, it can resolve empty vs. full AdV capsids and quantify their proportions (Fig. 1).
Its speed and excellent contrast resolution make the KaritroMP the only instrument on the market that can quickly measure AdV empty-full ratios.
KaritroMP analysis is ideal for comparing vector purification methods. The instrument can be used, for example, to monitor the effectiveness of purification during LVV production (Fig. 2).
Figure 2. The KaritroMP assesses the effectiveness of LVV sample purification processes. A sample analyzed before and after purification showed a ten-fold increase in the percentage of LVV capsids, corresponding to an increase in the infectious titer. The LVV samples were manufactured by Revvity Gene Delivery (formerly SIRION Biotech). For full details, download the product summary.
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