With mass photometry, you can precisely measure the empty/full capsid ratio for AAVs of any serotype in a matter of minutes. It requires only very small sample volumes and concentrations, and minimal sample preparation.
The SamuxMP precisely measures the empty/full capsid ratio for AAVs of any serotype as well as the quantities of partially filled and overfilled capsids. It can also detect impurities and aggregation, and give a rough capsid titer estimate as part of the standard data analysis procedure.
Empty/full AAV ratio measurement
Serotype-independent analysis
Partially filled and overfilled capsid quantification
Rough estimation of titer
< 5 minutes to result
Little sample needed
Minimal sample preparation
Very low operating costs
User training < 1 day
Software customized for AAV analysis
Benchtop instrument, easy decontamination
Mass photometry is a bioanalytical technology that measures the mass of individual AAV particles in solution, quickly and using small amounts of sample.
Mass photometry is ideally suited for measurements of empty/full capsid ratios. Filled AAV capsids have greater mass than empty capsids, so they can be readily distinguished from empty capsids on mass photometry histograms (Fig 1).
Figure 1 Mass photometry measurement of AAV capsids (AAVDJ serotype) with SamuxMP. Two distinct peaks are visible: one with lower mass corresponding to empty capsids, and a second with higher mass corresponding to full capsids.
The SamuxMP mass photometer can measure AAV capsids of any serotype without the need for protocol adjustments.
Figure 2 shows SamuxMP measurements of empty AAV capsids of four different AAV serotypes.
For each sample, the mass photometry measurements with the SamuxMP revealed a single, symmetric peak at the expected mass, confirming that the SamuxMP produces consistent results across serotypes.
Figure 2 Mass photometry measurements of empty AAV capsids of different serotypes on the SamuxMP. Mass histograms for four different AAV serotypes, with the mass and standard deviation calculated from three technical replicates.
Yes, the SamuxMP can distinguish empty, partially filled and full AAV particles.
Yes, the SamuxMP has a virus titer estimation function in its analysis software.
Yes, we recommend a calibration at least once a day. We recommend performing a 1-point calibration with empty AAV capsids of known mass.
A software package compliant with FDA and EU GMP Annex 11 regulations is available for use with the SamuxMP and SamuxMP Auto. In addition, the Refeyn service teams provide installation and operational qualifications (IQ/OQ) for instruments destined to GMP-regulated environments.
The SamuxMP Auto is the automated mass photometer for AAV characterization, autonomously measuring up to 24 samples in approximately 90 minutes. The instrument rapidly measures AAV empty/full ratios, sample purity and particle aggregation for any AAV serotype – with high precision and reproducibility.
Read this application note and learn how the SamuxMP mass photometer can quantify the empty/full capsid ratio of AAV samples in downstream purification, while also detecting impurities. Discover the advantages of the SamuxMP, in comparison to the gold-standard techniques such as analytical ultracentrifugation (AUC) and cryogenic transmission electron microscopy (cryoTEM), including its low sample and time requirements.
Read this application note and learn how the SamuxMP mass photometer can measure relative abundance of empty, full as well as heterogeneously loaded viral capsids in an AAV sample. Discover how it compares with analytical ultracentrifugation (AUC) in determining the relative abundances of each AAV population. Mass photometry measurements take only minutes, require minimal training and very small sample volumes, which makes the technology optimal for AAV analytics.
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