Mass photometry characterizes samples containing detergents
Membrane protein extraction and solubilization
Membrane proteins fulfill vital biological roles, representing a significant portion of potential therapeutical targets for a variety of diseases. As studying their native behavior is important, detergents and other membrane mimetics can be used for membrane protein solubilization prior to functional or structural studies.
Detergents are widely used for membrane protein extraction and throughout the protein purification process – detergents can also act as membrane mimetics for membrane protein solubilization. However, their presence in samples can interferes with some analytical techniques.
Characterizing membrane protein samples that include detergents
Mass photometry can measure samples containing detergents, eliminating the need for complete detergent removal. It works with a wide range of buffers, including those used for membrane protein extraction and solubilization.
As measurements take less than 5 minutes and readily detect protein aggregation, it is easy to characterize membrane protein samples. Moreover, mass photometry can detect detergent micelles, which makes it possible to determine the critical micelle concentration (CMC) of detergents under different conditions
To learn how mass photometry interacts with commonly used detergents
Mass photometry with detergents
For more information on analyzing membrane proteins with mass photometry
More Application Notes
Browse through our catalogue of application notes highlighting some recent case studies featuring mass photometry.