Comparing analytical approaches for AAV characterization
AAV sample characterization is a critical step in research, development and manufacturing processes of gene therapies involving these viral vectors (Figure 1). There are several analytical approaches available to assess critical quality attributes (CQAs) for AAV samples, such as capsid content (empty/full ratios) and titer.
Comparing mass photometry and CDMS for AAV analysis
Assessing AAV capsid content is critical for AAV research, development and production. There is an urgent need for reliable techniques that can characterize AAV samples efficiently and accurately, particularly when AAV sample loading shows a high level of heterogeneity [1].
Response to article on mass photometer stage alignment (J Pharm Sci, Nov 2022)
In the article Critical Calibration of Mass Photometry for Higher-Mass Samples Such as Adeno-Associated Virus Vectors (published this month in the Journal of Pharmaceutical Sciences [1]), the authors describe an issue they encountered with their Refeyn OneMP Mass Photometer.
The hows, whats and whys of automated mass photometry
The release of the TwoMPAuto in early 2022 represents an important improvement in the capabilities of Refeyn’s mass photometers. And like with any new product, there are some questions that frequently come up about how it works and what it can do.
Studying protein-DNA interactions with mass photometry
The study of protein-DNA interactions feeds into understanding the mechanisms of DNA replication, repair and transcription, gene expression, and the packaging of chromosomal DNA [1]. Now, a relatively new way to study protein-DNA interactions is entering the analytical toolbox: mass photometry.
How to study membrane proteins with mass photometry
The biologically important membrane proteins remain a challenging target for structural and functional studies, despite recent advances in biomolecular characterization capabilities. This is due to intrinsic difficulties in expressing, purifying and preparing membrane proteins for analysis while preserving their structure and activity.
Exploring retromer complex interactions
Q&A with Refeyn Travel Grant winner Kevin Chen
Mass photometry saves you time and money in cryo-EM experiments
You can never get enough time on an electron microscope. To make the most of the time you do have, you need to streamline the cryo-EM workflow. Negative stain EM (NS-EM) can help you optimize buffer conditions and assess sample quality before you move to the low-temperature microscope, but it’s a time-consuming, expensive technique. What you need is a cost-effective method that can speed up your analysis. The TwoMP, a bioanalytical tool that uses mass photometry to characterize biomolecules, can help you optimize your sample analysis without breaking the bank (or project budget). In this blog, Refeyn product manager Cathryn Langley, DPhil, tells us all about it.
Everything you need to know about analysing AAVs with mass photometry
Ask any AAV researcher – figuring out how many of your capsids are filled and how many are empty is a long and laborious process. There is a clear need to overcome the drawbacks of the current gold-standard analytical methods so users can rapidly and cost-effectively characterise AAVs.
Analyzing complex SARS-CoV-2 samples with mass photometry
The SARS-CoV-2 pandemic has altered our lives and shaped the course of scientific research, bringing an explosion in studies on the virus. Mass photometry has proved valuable in coronavirus research, especially in characterising complex and heterogeneous proteins, and in analysing protein interactions and stoichiometry. In this blog post, we highlight ways that mass photometry has helped investigate previously intractable questions and advance our understanding of SARS-CoV-2.
