Advances in Cell and Gene Therapy: Characterizing Large Viral Vectors Using Macro Mass Photometry
In this GEN webinar, Dr. Laura Pala will discuss how macro mass photometry enables fast, qualitative analysis of adenovirus and lentivirus samples for cell and gene therapies. She will introduce KaritroMP, a novel analytical instrument that uses macro mass photometry to characterize large viral vectors in research and development environments. During the webinar, she will […]
Tau protein aggregation & neurodegenerative disease
Join this webinar to discover how a single-molecule technology mass photometry has played a role in the understanding of tau protein aggregation, which is involved in neurodegenerative diseases like Alzheimer’s and Parkinson’s. Learn about the coexistence of native and misfolded conformers and their impact on aggregation kinetics, unlocking new insights into tau aggregation mechanisms. Attend […]
Studying lipoprotein lipase oligomerization dynamics with cryoEM and mass photometry
In this webinar, the speaker will describe experiments that uncovered how LPL interacts with its triglyceride substrate and what governs its oligomeric states. Cryogenic electron microscopy (cryoEM) was key to solving the structure of a dimeric LPL oligomer, while mass photometry facilitated a better understanding of LPL dimerization.
Harnessing mass photometry for AAV sample characterization in GMP-regulated environments
This webinar, will explain how the new, compliant software solutions address all regulatory requirements, including user management, audit trails, and signing options when exporting files from Refeyn software applications. The new software solutions will be compatible with the SamuxMP and SamuxMP Auto mass photometers, which are optimized for AAV analytics. This allows the ability to […]
創薬および製薬に向けたMass photometry法のアプリケーション
近年バイオ医薬品の開発が目まぐるしく、さらにそのモダリティ開発に関して積極的に取り組まれる中、分子生物学的にそのメカニズムをとらえていく際に蛋白質の挙動を確認していくことは非常に重要である。一方、抗体を代表とする蛋白質治療薬やAAVなどを利用した遺伝子治療薬においては、生物由来の材料を製造していくにあたり、物性的に均一な商品を産生する必要である。ここで示した2例は一見異なるように思えるが、分析化学の観点からみると、蛋白質やその周辺材料のサイズ分布を確認することが必要とされることが示される。今回の発表では、Mass photometry法の特徴を延べ、創薬および製薬分野においてのアプリケーション、特に分子間相互作用解析への応用例、他原理の補完的データ取得についての手法を紹介する。
Navigating The Complexities of AAV Scale Up to Keep Pace with the Increasing Demand For these Viral Vector Delivery Systems
In this webinar you will learn about the presence of inactive, empty capsids in potentially large quantities is one of the main challenges in AAV production. Quantifying the ratio of empty / full AAV capsids is currently challenging – existing methods are not quick and simple enough to be employed throughout the process.
Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis
In this webinar, Dr. John Zinder, a Senior Scientist in the Structural Biology group at Odyssey Therapeutics, will discuss the purification of shelterin subcomplexes and reconstitution of the entire complex using full-length, recombinant subunits. By combining mass photometry, negative-stain electron microscopy (EM), crosslinking mass spectrometry (XLMS), AlphaFold modelling, and native mass spectrometry (MS), stoichiometries were […]
Webinar | Monitoring Aggregation Levels of Biosimilar mAbs Using Mass Photometry and SEC
In the presented analysis, both SEC and mass photometry were used to measure aggregation levels. The results of this short study demonstrate that SEC and mass photometry are complementary, highlighting the usefulness of mass photometry as an orthogonal technique for monitoring aggregation of biopharmaceuticals such as mAbs.
Webinar | Assessing AAV production variability with single-particle mass analysis
This webcast will demonstrate how the mass of AAV capsids can be determined using two different novel, emerging single-particle techniques: charge-detection mass spectrometry (CDMS) and mass photometry. In contrast to ensemble-based approaches (e.g., native mass spectrometry), mass photometry and CDMS can measure the mass of numerous, individual AAV particles, circumventing issues driven by sample heterogeneity.
Webinar | Studying protein polymerization with mass photometry
Protein polymerization underpins major biological processes, such as cell division and intracellular transport, while undesired assembly can lead to protein aggregates that are associated with, for example, neurodegenerative diseases and cancer.
