AAV6 Upstream Empty/Full Capsid Optimization Using CIM SO3 Multi-Well Plates and Mass Photometry
This session will highlight new research focused on optimizing AAV6 upstream production – an essential step in improving the safety and affordability of gene therapies. Using a design of experiment (DoE) approach, the Sartorius BIA Separations team systematically identified the optimal conditions to achieve a high full capsid ratio. Further optimization was conducted with the Ambr15 system, while CIM SO3 monolithic plates enabled efficient high-throughput purification of upstream samples. Mass photometry was used to accurately assess capsid species ratios, supporting more precise and scalable production.
By attending this webinar, you will learn:
- How CIM SO3 plates can be utilized for high-throughput purification of AAV upstream samples where certain purity for analytics is needed.
- How best production conditions are determined to achieve the highest empty full capsid ratio using mass photometry.
- How to speed up your production process using Ambr15.