With its high sensitivity, the TwoMP is ideally suited for measurements at physiological (i.e. low) concentrations. The high dynamic range intrinsic to single molecule counting techniques ensures low-abundance species are still captured accurately.
Refeyn’s software automatically controls the acquisition process and performs the mass analysis in minutes. You can intuitively interpret the mass distribution results without the need for any prior knowledge.
A compact, bench-top instrument with minimal installation requirements. Measurements only require a few microliters of sample and clean sample-carrier slides.
Many proteins adopt a particular oligomeric form under certain conditions. In Figure 1, mass photometry characterises four different proteins: protein A, beta-amylase, urease and thyroglobulin. Through this characterisation, these proteins showcase a range of behaviours – from purely monomeric (as in Protein A) to a dynamic equilibrium between multiple states.
Mass photometry can be used to characterise DNA-protein complexes, which are crucial in gene expression, replication and DNA repair.
In this example, mass photometry not only allows detection of DNA binding but also provides information on how the oligomeric state of the protein changes upon DNA binding (Figure 2).
Ribosomes are macromolecular assemblies of protein and RNA, and are central sites for protein synthesis. Bacterial ribosomes (in this example from E. coli) are >2 MDa in size. Magnesium ions play a subtle yet important role in the assembly of intact (70S) complexes. In the absence of magnesium, E. coli ribosomes rapidly disassemble into their 30S and 50S subunits. Mass photometry allows us to monitor these processes (Figure).
The TwoMP has a wide mass range, allowing the study of different biomolecules, including proteins, DNA, RNA, large macromolecular complexes, nanostructures and even small viruses such as AAVs.
The only optimization step necessary prior to a mass photometry measurement is to adjust the final concentration of the sample; the sample concentration on the instrument should be between 100 pM and 100 nM. The optimal concentration range for a mass photometry measurement is 5-20 nM.
For biomolecules within the mass detection range of the TwoMP (30 kDa to 5 MDa), the shape/conformation doesn’t influence the interferometric contrast and thus mass measured by the mass photometer.
In mass photometry, the mass measurement depends on the interference between the light scattered by the molecule and reflected light from the glass surface. This interference signal will be orders of magnitude greater than the fluorescence signal, so the mass measurement will not be affected.
The Refeyn TwoMP brings mass photometry into daily laboratory life. This unique instrument allows you to characterise your molecule of interest with unprecedented sensitivity, speed and simplicity. Learn more about its specifications by downloading the product data sheet.
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