Assessing AAV stability with mass photometry: Striking insights into the outcomes of heated capsids
In this webinar, we demonstrate how the mass of AAV capsids can be determined using two different novel, emerging single-particle techniques: Charge-detection mass spectrometry (CDMS) and mass photometry. In contrast to ensemble-based approaches (e.g. native mass spectrometry), mass photometry and CDMS can measure the mass of numerous, individual AAV particles, circumventing issues driven by sample […]
Innovative Upstream Process Analytical Technologies for Enhanced AAV Manufacturing
Adeno-associated viruses (AAVs) play a crucial role in gene therapy, but producing high-quality vectors at scale remains challenging, particularly due to inefficiencies in DNA packaging. This results in a high proportion of empty or improperly loaded capsids. To address this, Refeyn, Rentschler Biopharma, and the Cell and Gene Therapy Catapult, with support from Innovate UK, […]
De Novo Design of Allosterically Switchable Protein Assemblies
Designing nanodevices that can change shape and function in response to molecular signals is a key objective in protein design, paving the way for the development of switchable nanomaterials, triggerable drug-delivery systems, and molecular motors. In this talk, I will present recent progress in designing and characterizing a diverse range of switchable protein assemblies.
Evaluating Analytical Strategies to Quantify Capsid Titre: Towards a Platform-Method Approach to Accelerate AAV Drug Product Development
Traditional capsid titre methods rely on ELISA which commonly suffers from long turnaround times, low throughput, and large volume sample requirements. This limits the application of ELISA-based methods to routine analysis, thus requiring development of alternative high-throughput (HTP) capsid titre methods. We have performed a comprehensive assessment on currently available orthogonal capsid titre methods using […]
Teach Me in 10: Accelerating Cell and Gene Therapy Development
In this 10-minute session, we’ll show you how mass photometry is transforming AAV production with fast, reproducible measurements that use minimal sample at less than $5 per measurement. Mass photometry is also highly user-friendly, with a benchtop instrument that make it perfect for in-house use, cutting reliance on external facilities and boosting research efficiency without […]
Leveraging mass photometry for efficient, precise, and rapid adeno-associated virus (AAV) empty:full quantification
This webinar is the second in a series covering our Challenge Themes and the collaborative research projects that are helping us to identify effective solutions to the challenges facing the advanced therapies sector. Here, we will discuss how we are working with a collaborator to drive efficiency of Adeno-Associated Virus (AAV) production and quantification.
Quickly and accurately measure key analytical attributes of viral vectors with macro mass photometry
In this webinar, Dr. Laura Pala explains how the Karitro MP macro mass photometer measures the size and contrast (related to mass) of viral vectors at the single-particle level, enabling characterization of purity, populations, and physical titer of viral vector samples with speed, accuracy and no need for labels or other sample modification. Through case […]
Using Mass Photometry To Power Up the Development of AAV-Based Therapeutics
In this webinar, hear how mass photometry, an easy-to-use bioanalytical technology, can characterize AAV samples in minutes and using little sample. Our expert speaker will also discuss how mass photometry can be readily integrated throughout development and manufacturing.
Measuring viral vector purity, stability and physical titer in minutes – with just one tool
In this webinar, Dr. Laura Pala will discuss a new technology that enables fast, accurate and label-free analysis of adenovirus (AdV) and lentivirus (LV) samples for cell and gene therapy R&D. This technology – macro mass photometry – characterizes samples of large viral vectors in minutes, at a single-particle level. Dr. Pala will introduce the […]
Characterizing oligomerization dynamics of a ubiquitin ligase using mass photometry
This webcast will demonstrate harnessing the power of mass photometry to analyze the oligomeric state of the DCAF15–DDA1–DDB1 E3 ligase at low nanomolar concentrations. This study revealed, for the first time, the formation of dimers and trimers with quantified dissociation constants. The oligomeric remodelling effect emphasized the transition from trimer to monomer upon molecular glue […]
