Enabling early AAV characterization: the role of mass photometry in upstream process development
Join us for a webinar to learn how advances in purification techniques have enhanced AAV sample compatibility with mass photometry in upstream development, delivering real-time insights for improved process control and faster decision-making. Attendees will see pre- and post-cleanup case study data provided by Refeyn’s collaborators, Généthon and Rentschler, in addition to mass photometry-supported data […]
Derisk early and accelerate antibody development with mass photometry -column-free aggregation assessment and in solution binding mechanisms
Join me to discover how integrating mass photometry into antibody development workflows can drive rapid reliable decision-making. Mass photometry enhances antibody development workflows with insights from: Column-free aggregation assessment in minutes using only nanograms of sample In solution assessment of antibody-antigen binding mechanisms from nM and uM concentration samples Streamlined methodology consisting of only one […]
Assessing mRNA stability and integrity using mass photometry
We will present quantitative results from inhibitor binding studies using mass photometry. Attendees will find out how to implement mass photometry-based workflows for rapid assessment of RNA integrity, enabling faster troubleshooting and quality checks in RNA production or research. Adopting a label-free strategy for monitoring mRNA stability reduces reliance on traditional, time-consuming assays like gel […]
Webinar: USP standards to support AAV quality testing and analytical characterization of AAV content utilizing mass photometry and orthogonal techniques
This webinar explores the latest advancements and persistent challenges in AAV-based gene therapy, focusing on analytical testing and manufacturing control. Dr. Anthony Blaszczyk introduces the first USP AAV reference materials, developed to support consistent testing across the gene therapy lifecycle – from raw material qualification to product release. Additionally, Drs. Paul Getty and Lauren Tomlinson […]
Assessing AAV stability with mass photometry: Striking insights into the outcomes of heated capsids
In this webinar, we demonstrate how the mass of AAV capsids can be determined using two different novel, emerging single-particle techniques: Charge-detection mass spectrometry (CDMS) and mass photometry. In contrast to ensemble-based approaches (e.g. native mass spectrometry), mass photometry and CDMS can measure the mass of numerous, individual AAV particles, circumventing issues driven by sample […]
Innovative Upstream Process Analytical Technologies for Enhanced AAV Manufacturing
Adeno-associated viruses (AAVs) play a crucial role in gene therapy, but producing high-quality vectors at scale remains challenging, particularly due to inefficiencies in DNA packaging. This results in a high proportion of empty or improperly loaded capsids. To address this, Refeyn, Rentschler Biopharma, and the Cell and Gene Therapy Catapult, with support from Innovate UK, […]
De Novo Design of Allosterically Switchable Protein Assemblies
Designing nanodevices that can change shape and function in response to molecular signals is a key objective in protein design, paving the way for the development of switchable nanomaterials, triggerable drug-delivery systems, and molecular motors. In this talk, I will present recent progress in designing and characterizing a diverse range of switchable protein assemblies.
Evaluating Analytical Strategies to Quantify Capsid Titre: Towards a Platform-Method Approach to Accelerate AAV Drug Product Development
Traditional capsid titre methods rely on ELISA which commonly suffers from long turnaround times, low throughput, and large volume sample requirements. This limits the application of ELISA-based methods to routine analysis, thus requiring development of alternative high-throughput (HTP) capsid titre methods. We have performed a comprehensive assessment on currently available orthogonal capsid titre methods using […]
Teach Me in 10: Accelerating Cell and Gene Therapy Development
In this 10-minute session, we’ll show you how mass photometry is transforming AAV production with fast, reproducible measurements that use minimal sample at less than $5 per measurement. Mass photometry is also highly user-friendly, with a benchtop instrument that make it perfect for in-house use, cutting reliance on external facilities and boosting research efficiency without […]
Leveraging mass photometry for efficient, precise, and rapid adeno-associated virus (AAV) empty:full quantification
This webinar is the second in a series covering our Challenge Themes and the collaborative research projects that are helping us to identify effective solutions to the challenges facing the advanced therapies sector. Here, we will discuss how we are working with a collaborator to drive efficiency of Adeno-Associated Virus (AAV) production and quantification.
