Upstream AAV characterization with mass photometry
This application note and protocol describe a simple, plate-based cleanup method for clarified lysates, plus mass photometry. This approach enables analysis of AAV quality during upstream processing. Taking just 3 hours from bioreactor to results and using only 1.5 mL of cell culture sample, it measures % empty, partially filled and full capsids. Applicable for multiple serotypes, it makes process optimization faster and more cost-effective than traditional post-purification checkpoint control.
