ON-DEMAND WEBINAR
Combining icIEF fractionation and mass photometry to explore bispecific charge variants and aggregation
Presenters: Dr. Srinivasa Rao (Bio‑Techne) and Dr. Nina Jajcanin Jozic (Refeyn)
How can you gain deeper insight into bispecific charge variants and aggregation –using less sample and less time?
Our on‑demand webinar introduces a powerful, streamlined workflow for characterizing complex antibody modalities.
In this session, Dr. Srinivasa Rao (Bio‑Techne) and Dr. Nina Jajcanin Jozic (Refeyn) showcase how imaged capillary isoelectric focusing (icIEF) fractionation can be integrated with label‑free mass photometry to deliver more comprehensive charge–size variant analysis.
Using the MauriceFlex™ System alongside Refeyn’s TwoMP™ mass photometer, the speakers demonstrate a fast and intuitive application that combines charge variant separation and fractionation with downstream charge–size analysis. This approach highlights clear differences between a bispecific antibody (Mosunetuzumab‑axgb) and a research‑grade biosimilar.
The webinar demonstrates how this combined workflow enables:
- Direct tracking of LMWS and HMWS across acidic, main, and basic charge variants
- Characterization under both control and forced degradation conditions
- Actionable insights from only nanogram‑level sample quantities
The results provide valuable insight into how bispecific charge variants and aggregation behavior change under different conditions. Together, these two compact, user‑friendly instruments offer a time‑saving, straightforward solution for advanced charge variant characterization of complex biomolecules.
