ON-DEMAND WEBINAR
Combining icIEF fractionation and mass photometry to explore bispecific charge variants and aggregation
Presenters: Dr. Srinivasa Rao (Bio‑Techne) and Dr. Nina Jajcanin Jozic (Refeyn)
For the first time, researchers can directly measure size and aggregation across bispecific charge variant fractions.
Conventional tools require too much sample to measure charge and size variants together, but the workflow introduced in this on-demand webinar makes it possible. By combining icIEF fractionation on Bio-Techne’s MauriceFlex™ System with mass photometry on Refeyn’s TwoMP mass photometer, researchers can directly correlate charge variants with size and aggregation profiles – in around four hours.
This capability has particular significance for biosimilar development, where demonstrating comparability to an originator molecule depends heavily on charge variant analysis. The ability to directly assess size and aggregation across those fractions adds a new and previously inaccessible dimension to comparability data.
Dr. Srinivasa Rao (Bio-Techne) and Dr. Nina Jajcanin Jozic (Refeyn) demonstrate the workflow using Mosunetuzumab-axgb and a research-grade biosimilar, showing how size variants distribute across acidic, main, and basic charge variant fractions under both control and forced degradation conditions. This information is not accessible using conventional approaches, such as size-exclusion chromatography (SEC), because they require far more sample than a single round of icIEF fractionation yields.
The webinar demonstrates how this combined workflow enables you to:
- Generate direct comparability evidence for biosimilar development
- Correlate charge variant profiles with size data in a single, four-hour workflow
- Track low- and high-molecular-weight species, including aggregates, across charge variants under control and stress conditions
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