Assess protein sample purity and homogeneity in minutes with mass photometry using only nanograms of sample.
Know that your sample is ready. The Refeyn MyMass™ mass photometer (MyMassMP) gives you fast, accurate assessment of purity and homogeneity – so you can proceed to cryo-electron microscopy (cryo-EM), surface plasmon resonance (SPR), or biolayer interferometry (BLI) with confidence.
MyMass makes it easy to:
Verify sample purity
Assess sample homogeneity
Check oligomerization state
De-risk costly experiments
Who uses MyMassMP
In structural biology, every experiment counts. MyMass is designed for structural biologists and the teams that support them – to give them the clarity and efficiency they need so they can focus on discovery. MyMass is built for researchers who are regularly:
Purifying proteins
MyMass confirms purity and homogeneity
Gain insight from single-molecule resolution that bulk methods, like SEC, SDS-PAGE, or DLS, cannot match
Running high-value experiments
MyMass reveals poor sample quality before it can ruin cryo-EM, MS, SPR, or BLI experiments
Know your sample is ready before you commit – saving time, cost, and precious material
Screening and optimizing sample conditions
MyMass tests multiple conditions in minutes
Measurements are run in native buffer – no column interactions, no denaturation
Rapidly identify the preparation that is mostly likely to succeed
Key data
Figure 1. A single, one-minute measurement reports purity and oligomerization – In this MyMass interface of a thyroglobulin (Tg) measurement, the peaks correspond to contaminants (A & B), monomers (C), dimers (D) and tetramers (E).
Got a minute? That’s all it takes to find out if your sample is ready.
Specifications:
- Results in 1 minute. Fast enough to screen multiple conditions in a single session.
- 55 kDa – 3 MDa mass range. Suitable for a broad range of proteins and complexes.
±5% mass precision. Reproducible results, so you can make go/no-go decisions with confidence. - ±10% mass error. Accuracy with a single measurement – no repeats required.
- Ready to measure in 1 minute from power-on. Just walk up and measure.
Supporting Headline
Rapid turnaround
From power-on to results in just minutes
1 minute per measurement
Low sample consumption
30 µL at nM concentration
Single-molecule sensitivity reveals species at 5% abundance
In solution
Analysis in native conditions
Compatible with many buffers
MyMassMP instrument overview
Explore the main hardware components of the MyMassMP system
Everything you need, built in. Guided workflows walk you through each measurement step by step, and results are analyzed onboard – no PC required.
- Read in a minute
- Just walk up and measure.
- Dilute, pipette, measure.
- No complex sample prep – just load your cassette and go.
- Powerful optics, compact footprint.
- Proven technology in a single benchtop unit – 560 mm W x 375 mm D x 380 mm H)
Rear panel & connectivity
View the instrument ports and connectivity options.
- Export your way.
- USB-A and USB-C ports to transfer results directly from the instrument.
Network connectivity.
Connect to your network for flexible data sharing and IT integration.
Software that guides you from sample to result
MyMass features onboard software with a guided workflow. A touchscreen interface walks you through every step, with diagrams, videos, and built-in help so even occasional users can get results with confidence.
Guided workflows: Step-by-step instructions with diagrams and videos make MyMass simple to walk up and use
Simple, powerful analysis: Mass distribution, automatic peak fitting, and a clear results table give you everything you need to assess your sample
Built-in support: Extensive help, tools, and FAQs are always at hand
Frequently asked questions
What is the MyMass mass photometer?
The MyMass mass photometer, or MyMassMP, is a compact, benchtop instrument designed for quickly checking samples prior to cryo-EM, SPR, and other costly techniques. It uses mass photometry technology – an innovative single-molecule mass measurement technique. To learn more about mass photometry technology, read our article, How does mass photometry work?.
How can MyMassMP help with cryo-EM sample optimization?
Mass photometry measures the mass of single particles in solution and uses a histogram to report the mass distribution of the sample, providing an overview of all the populations in a sample. You can read more about how mass photometry works and learn more about interpreting mass photometry data.
Mass photometry helps determine sample purity and homogeneity. Collecting these data is an important step in sample quality control – improving structural resolution and data reproducibility.
What types of samples can MyMassMP analyze?
Proteins – including membrane proteins, protein complexes, and antibodies – within the supported mass range (55 kDa – 3 MDa).
What mass range does MyMassMP cover?
55 kDa – 3 MDa.
How much sample is required for MyMassMP measurements?
Sample consumption is very low (30 µL volume at 10 nM concentration is optimal).
Do MyMassMP measurements require labels?
No labeling (e.g. fluorescent or isotopic labeling) is required, as mass photometry is a label-free technique.
How long does a MyMass measurement take?
Just one minute. This is a powerful advantage, as it can save hours of experimental optimization time.
How big is a MyMassMP instrument?
MyMassMP is a compact, all-in-one instrument with an integrated touchscreen, and it fits on a lab benchtop.
How easy is MyMassMP to use?
It is designed for routine use by non-specialists, with a guided workflow via the integrated touchscreen.
How does mass photometry compare to other techniques?
Mass photometry has been shown to agree well with other techniques, including negative stain EM, charge detection mass spectrometry (CD-MS) and native MS, but mass photometry is much faster and simpler to use.
Do I need special buffers for MyMass measurements?
Mass photometry does not require specific buffers; it is compatible with the most widely used buffers. This wide compatibility, along with its low time and sample requirement, make mass photometry ideal for repeated testing to optimize buffer conditions.
What other instruments are available for mass photometry in structural biology?
In addition to MyMassMP, Refeyn offers the powerful TwoMP mass photometer, along with all the necessary consumables. If repeated analysis of large sets of sample is needed, there is an automated version of the TwoMP. For the analysis of low-affinity interactions that require higher concentrations, a microfluidics add-on – MassFluidix™ HC – is also available.
How does MyMassMP compare to other Refeyn mass photometers, such as TwoMP?
All Refeyn mass photometers (TwoMP, Samux™, and MyMassMP) feature mass photometry technology and fit on a lab benchtop. MyMassMP is designed for rapid sample checks and is an all-in-one instrument featuring an integrated touchscreen for standalone operation – making it the most compact. TwoMP, Refeyn’s flagship mass photometer, delivers unparalleled bioanalytical performance. With a slightly broader mass range, powerful analysis software, and more dedicated consumables, the TwoMP enables a broader range of applications, including analysis of complex protein and nucleic acid samples. SamuxMP is a powerful mass photometer optimized for the analysis of adeno-associated virus (AAV) samples.
MyMassMP consumables
*** Consumables for MyMass will be available soon. Stay tuned for updates! ***
- Instrument
Are you seeking mechanistic insights?
TwoMP has a wide mass range, powerful analysis software, and more dedicated consumables, allowing the study of interactions, oligomerization, stoichiometry and more.
Learn more about MyMass
Explore our mass photometry resources
Webinar: Cryo-EM screening with mass photometry
In this webinar, hear Dr. Adar Sonn-Segev describe how mass photometry provides quantitative information on sample heterogeneity with molecular resolution using minimal volumes.
How mass photometry saves time (and money) in cryo-EM experiments
This article explains how mass photometry offers a fast, low-sample, label-free way to assess sample quality before cryo-EM, helping you avoid poor grids and improve structural outcomes.
Enhancing cryo-EM workflows with mass photometry: A smarter pre-screening approach
From aggregation to oligomeric state, learn how mass photometry streamlines pre-cryo-EM screening so you can focus on high-quality structural data, not troubleshooting.
Ready to revolutionize your research?
Find out how mass photometry can transform your sample characterization. Whether you’re focused on drug development, discovery science, or manufacturing, Refeyn’s innovative technology provides the insights you need to accelerate your breakthroughs.
Shop MyMassMP consumables
MyMassMP - Alignment Kit (MP-CON-21030)
MyMassMP Alignment Kit streamlines protein analysis with MyMassMP for precise and reproducible results
MyMassMP - Sample Prep Kit (MP-CON-21031)
MyMassMP Sample Pre Kits enable reliable protein analysis on MyMassMP with clea and ready-to-use slides for reproducible results.
Assess sample purity and homogeneity in minutes, using nanograms of sample.
Refeyn’s MyMassTM mass photometer gives you the information you need when preparing samples for techniques such as cryo-electron microscopy (cryo-EM), surface plasmon resonance (SPR), and biolayer interferometry (BLI).
MyMassMP makes it easy to:
Check purity
Check homogeneity
Spot sample problems early
Stop wasting time and money running expensive assays on poor samples
