Assess sample purity and homogeneity in minutes, using nanograms of sample.
Refeyn’s MyMassTM mass photometer gives you the information you need when preparing samples for techniques such as cryo-electron microscopy (cryo-EM), surface plasmon resonance (SPR), and biolayer interferometry (BLI).
MyMassMP makes it easy to:
Check purity
Check homogeneity
Spot sample problems early
Stop wasting time and money running expensive assays on poor samples
Supporting Headline
Rapid turnaround
From power-on to results in just minutes
1 minute per measurement
Low sample consumption
30 µL at nM concentration
Single-molecule sensitivity reveals species at 0.1% abundance
In solution
Analysis in native conditions
Compatible with many buffers
- Instrument
Are you seeking mechanistic insights?
TwoMP has a wide mass range, powerful analysis software, and more dedicated consumables, allowing the study of interactions, oligomerization, stoichiometry and more.
Frequently asked questions
What is the MyMass mass photometer?
The MyMass mass photometer, or MyMassMP, is a compact, benchtop instrument designed for quickly checking samples prior to cryo-EM, SPR, and other costly techniques. It uses mass photometry technology – an innovative single-molecule mass measurement technique. To learn more about mass photometry technology, read our article, How does mass photometry work?.
How can MyMassMP help with cryo-EM sample optimization?
Mass photometry measures the mass of single particles in solution and uses a histogram to report the mass distribution of the sample, providing an overview of all the populations in a sample. You can read more about how mass photometry works and learn more about interpreting mass photometry data.
Mass photometry helps determine sample purity and homogeneity. Collecting these data is an important step in sample quality control – improving structural resolution and data reproducibility.
What types of samples can MyMassMP analyze?
Proteins – including membrane proteins, protein complexes, and antibodies – within the supported mass range (55 kDa – 3 MDa).
What mass range does MyMassMP cover?
55 kDa – 3 MDa.
How much sample is required for MyMassMP measurements?
Sample consumption is very low (30 µL volume at 10 nM concentration is optimal).
Do MyMassMP measurements require labels?
No labeling (e.g. fluorescent or isotopic labeling) is required, as mass photometry is a label-free technique.
How long does a MyMass measurement take?
Just one minute. This is a powerful advantage, as it can save hours of experimental optimization time.
How big is a MyMassMP instrument?
MyMassMP is a compact, all-in-one instrument with an integrated touchscreen, and it fits on a lab benchtop.
How easy is MyMassMP to use?
It is designed for routine use by non-specialists, with a guided workflow via the integrated touchscreen.
How does mass photometry compare to other techniques?
Mass photometry has been shown to agree well with other techniques, including negative stain EM, charge detection mass spectrometry (CD-MS) and native MS, but mass photometry is much faster and simpler to use.
Do I need special buffers for MyMass measurements?
Mass photometry does not require specific buffers; it is compatible with the most widely used buffers. This wide compatibility, along with its low time and sample requirement, make mass photometry ideal for repeated testing to optimize buffer conditions.
What other instruments are available for mass photometry in structural biology?
In addition to MyMassMP, Refeyn offers the powerful TwoMP mass photometer, along with all the necessary consumables. If repeated analysis of large sets of sample is needed, there is an automated version of the TwoMP. For the analysis of low-affinity interactions that require higher concentrations, a microfluidics add-on – MassFluidix™ HC – is also available.
How does MyMassMP compare to other Refeyn mass photometers, such as TwoMP?
All Refeyn mass photometers (TwoMP, Samux™, and MyMassMP) feature mass photometry technology and fit on a lab benchtop. MyMassMP is designed for rapid sample checks and is an all-in-one instrument featuring an integrated touchscreen for standalone operation – making it the most compact. TwoMP, Refeyn’s flagship mass photometer, delivers unparalleled bioanalytical performance. With a slightly broader mass range, powerful analysis software, and more dedicated consumables, the TwoMP enables a broader range of applications, including analysis of complex protein and nucleic acid samples. SamuxMP is a powerful mass photometer optimized for the analysis of adeno-associated virus (AAV) samples.
Learn more about MyMass
Explore our mass photometry resources
Webinar: Cryo-EM screening with mass photometry
In this webinar, hear Dr. Adar Sonn-Segev describe how mass photometry provides quantitative information on sample heterogeneity with molecular resolution using minimal volumes.
How mass photometry saves time (and money) in cryo-EM experiments
This article explains how mass photometry offers a fast, low-sample, label-free way to assess sample quality before cryo-EM, helping you avoid poor grids and improve structural outcomes.
Enhancing cryo-EM workflows with mass photometry: A smarter pre-screening approach
From aggregation to oligomeric state, learn how mass photometry streamlines pre-cryo-EM screening so you can focus on high-quality structural data, not troubleshooting.