Accelerating chemical cross-linking optimization with denaturing mass photometry
Presenter: Dr. Oscar Hernandez Alba, Research Scientist at the Bioorganic Mass Spectrometry Laboratory, CNRS
Chemical cross-linking (XL) has become an increasingly valuable tool for studying protein–protein interactions (PPIs), including transient assemblies and low-abundance subcomplexes.
However, identifying optimal cross-linking reagents and reaction conditions remains a significant challenge, often relying on time-consuming workflows and analytical methods that provide limited quantitative insight.
As the demand grows for more efficient and informative approaches to protein characterization, researchers are seeking technologies that can simplify XL optimization while delivering robust, quantitative data.
In this on-demand webinar, Dr. Oscar Hernandez Alba, introduces denaturing mass photometry (dMP) and demonstrates how it can be used to rapidly assess and optimize chemical cross-linking workflows. Through real-world examples, the session highlights how dMP enables direct characterization and quantification of cross-linked species, helping researchers make more informed decisions before downstream mass spectrometry analysis.
What you’ll learn?
Discover how dMP enables:
- Rapid and irreversible protein denaturation – achieving 95% denaturation in just 5 minutes
- Accurate mass measurements across a broad dynamic range from 30 kDa to 5 MDa
- Direct, label-free quantification of all coexisting cross-linked species, including target complexes, intermediates, and aggregates
- Faster optimization of cross-linking conditions ahead of downstream mass spectrometry analysis