Comparing mass photometry and SEC for antibody aggregation assessment

Mass photometry is an emerging tool for assessing multiple antibody attributes, including aggregation. In this app note, a comparison to size-exclusion chromatography (SEC) shows the two techniques agree, but mass photometry has superior resolution,  particularly for larger modalities. Mass photometry requires no optimization beyond sample dilution and readily characterized samples that could not initially be measured with SEC. In addition, mass photometry requires >100x less sample and is 20x faster than SEC, and provides an informative mass readout. The app note was created in collaboration with Absolute Antibody.