SizeFerence™ Calibrant

Ref: MP-CON-41030

Top Features of SizeFerence

  • Delivers dependable calibration for macro mass photometry measurements of particles within the 40–150 nm range, including adenoviruses and lentiviruses
  • Incorporates silica beads of multiple diameters (50, 60, 80, 100, and 120 nm) for accurate size and contrast calibration using the KaritroMP instrument
  • Guarantees standardized and reproducible measurements across various instruments and laboratories with user-friendly calibration

Top Applications of SizeFerence

  • Calibration of macro mass photometry measurements for precise particle size and contrast determination
  • Facilitates viral vector characterization, including adenoviruses and lentiviruses

$2,500.00

SizeFerence is a calibrant designed for the KaritroMP macro mass photometer, ensuring accuracy in particle size and contrast measurements within the 40–150 nm range. This includes critical particles such as adenoviruses, lentiviruses and other viral vectors. The calibrant features silica beads of known diameters (50, 60, 80, 100, and 120 nm), serving as reference points for particle size and contrast determination. These beads are mixed in roughly equimolar concentrations and automatically analyzed by the KaritroMP instrument to develop a model that calculates the particle size and contrast of the sample.

Macro mass photometry operates by measuring two essential parameters for each particle: contrast, which acts as a proxy for mass, and particle size, determined by identifying the optimum contrast point after conducting a focus sweep of the sample. This process is possible thanks to an automatic calibration with SizeFerence, standardizing measurements across various experiments, instruments, and laboratories.

SizeFerence is user-friendly and ensures consistent calibration, guaranteeing reproducibility in measurements across diverse settings. It is available in a 0.3 mL suspension of silica beads in deionized water and requires dilution before use, following the batch-specific instructions provided.
For optimal performance, SizeFerence should be stored at room temperature (maximum 25°C) and remains stable for up to one year. It should not be frozen. Our expert support team is always available to assist you, ensuring you achieve the best results with our cutting-edge products.

Additional information

Brand Refeyn
Pack Size 1
Contents Silica beads suspended in deionized water (0.3 mL) For calibration of non-reflective particles in the 40–150 nm range measured with the Karitro™️
Number of Experiments Conducted A single vial allows > 300 calibrations (depending on the batch)
Reusable No
Instrument Compatibility KaritroMP
Volume in Tube 0.3 mL
Sample Volume for Use Instructions provided with the product
Storage Instructions Room Temperature
Shipment Conditions Room temperature
Shelf Life 12 months
Shelf Life After Opening See Instruction provided with the product

Why is SizeFerence calibrant required in every KaritroMP acquisition run?

Each run requires a single calibration, as SizeFerence provides a fundamental reference for particle size and contrast. Using silica beads with known diameters and contrast as reference markers, SizeFerence guarantees standardized and reproducible measurements across different instruments, operators, and laboratories, which our analysis software automatically handles.

How to use SizeFerence?

SizeFerence must be diluted before use. Please adhere to the instructions provided with the product, optimized for each batch.