$279.00
MassFerence P1 is a specialized calibration standard developed by Refeyn to enhance the accuracy and consistency of mass photometry measurements for proteins. Designed to calibrate measurements of proteins with molecular masses ranging from 90 to 1000 kDa on OneMP and TwoMP and the full mass range for MyMassMP, it is well-suited for a broad spectrum of protein-related applications in research and development. The MassFerence P1 encompasses a standard protein with several oligomeric forms ranging from 86 to 344 kDa, providing exceptional versatility for a wide range of protein samples.
Mass photometry works by measuring the interference between the light scattered by individual biomolecules and the light reflected from the measurement surface. This interference correlates directly with the mass of the biomolecules, allowing researchers to accurately determine the sample’s mass. To ensure precise mass measurements, calibrants with known mass serve as references. MassFerence P1 provides a standardized, reliable reference to optimize contrast-to-mass conversion.
Refeyn’s MassFerence P1 is designed for ease of use, providing a convenient solution for protein calibration. It is ready to use, straight out of the box, requiring only a simple dilution. The use of Massference P1 enables faster data analysis workflows in our software. The standard is compatible with a wide array of standard buffers, ensuring adaptability across various experimental conditions.
Each box of MassFerence P1 includes three 50 µL aliquots, offering ample material for multiple uses. This ensures that researchers can consistently replicate their experiments across different laboratories and instruments, significantly enhancing the reliability and reproducibility of their data. Whether engaged in academic research, drug development, or protein characterization, MassFerence P1 stands as an invaluable tool for researchers employing mass photometry.
| Brand | Refeyn |
|---|---|
| Type | Calibrant |
| Pack Size | 1 |
| Contents | 1 box containing 3 x 50 µL aliquots |
| Mass Range | 90 to 1000 kDa (TwoMP, OneMP) 55kDa to 3000 kDa (MyMassMP) |
| Number of Experiments Conducted | Each vial is sufficient for 10 days of calibrations |
| Reusable | No |
| Instrument Compatability | OneMP, TwoMP, TwoMP Auto, MyMassMP |
| Sample Compatibility | Any antibody, protein or protein complex in the recommended mass range |
| Volume in Tube | 50 µL aliquots |
| Sample Volume for Use | Aliquot 10 x 5µL upon first thaw for best results – see product data sheet for more information |
| Storage Instructions | Store at −20°C; stable for up to 6 months Upon first thaw, aliquot into 10 × 5µL portions to maintain performance Do not store at 4°C or on ice at any point The working solution may be kept at room temperature for up to 1 day |
| Shipment Conditions | Dry ice |
| Shelf Life | 6 months after manufacturing, at -20°C |
| Shelf Life After Opening | See Instructions on Product Data Sheet |
MassFerence P1 is engineered to deliver maximal precision for protein measurements on OneMP, TwoMP, and MyMassMP systems. For OneMP and TwoMP mass photometers, MassFerence P1 provides high precision for molecular weights ranging from 90 kDa to 1000 kDa, maintaining mass accuracy of less than 5%. For MyMassMP, MassFerence P1 covers the complete mass range (55 kDa to 3000 kDa), maintaining mass accuracy of less than 10%. A key advantage is its automatic calibration capability integrated into our analysis software, improving consistency across experiments, instruments, and laboratories. This ensures calibration processes remain optimized for mass photometry.
MassFerence P1 is the recommended calibrant for OneMP and TwoMP, designed and validated to deliver results within specifications (<5% mass accuracy) for proteins ranging from 90 to 1000 kDa. For MyMassMP, it covers the full mass range while maintaining mass accuracy under 10%. MassFerence P1 is supplied in a box containing three 50 µL vials. Refeyn advises aliquoting each vial upon first thaw to maximize long‑term stability and minimize freeze‑thaw cycles that could affect product quality. Never store MassFerence P1 at 4 °C or on ice. The working solution should remain at ambient temperature for a maximum of one day. For detailed usage instructions, refer to the Product Data Sheet for MassFerence P1 (MFP1 PDS).
No. The working solution (1:500 dilution from stock) is stable at room temperature for 24 hours in PBS. Never store MassFerence P1 on ice or at 4 °C.
Outside these conditions, the calibrant is not stable enough to maintain the oligomeric states required for proper calibration.
No. Refeyn recommends preparing 5 µL aliquots upon the first thaw of each vial to limit freeze‑thaw cycles and ensure optimal performance.
The TwoMP has a mass range from 30 kDa to 5 MDa. For the low‑mass range below 90 kDa, Refeyn recommends using a commercially available protein with a molecular weight similar to your protein of interest. If the target masses span both below and above 90 kDa, we recommend performing a two‑step calibration using MassFerence P1 with a low‑molecular‑weight protein. Additional guidance is available in the user manuals.
MassFerence P1 is compatible with various standard buffers, but the oligomeric distribution may shift depending on the buffer used. Such variations may alter peak counts but do not affect calibration accuracy. On OneMP and TwoMP, for optimal calibration, select three or more peaks with >100 counts each, or use the automatic calibration feature by selecting MassFerence P1” in AcquireMP when saving the acquisition. On MyMassMP, the process of calibration is automatic. An error message will appear if calibration is suboptimal. In this case, follow the on‑screen guidance and refer to the integrated FAQ on the instrument for additional support.
The TwoMP and MyMassMP instruments have different mass ranges and precision levels, which determines how MassFerence P1 performs on each system. The TwoMP measures from 30 kDa to 5 MDa, but MassFerence P1 is recommended specifically for 90 kDa to 1 MDa, where it achieves <5% mass accuracy and therefore the best performance. The MyMassMP supports a broader 55 kDa to 3000 kDa range, but with a different precision profile—MassFerence P1 maintains <10% mass accuracy across this full range. Because of these differences in both mass range and precision, MassFerence P1 is suitable for the entire validated mass range of MyMassMP, while its optimal performance window on TwoMP is narrower.
