$275.00
MassFerence P1 is a specialized calibration standard developed by Refeyn to enhance the accuracy and consistency of mass photometry measurements for proteins. Designed to calibrate measurements of proteins with molecular masses ranging from 90 to 1000 kDa, it is well-suited for a broad spectrum of protein-related applications in research and development. The MassFerence P1 encompasses a standard protein with several oligomeric forms that range in molecular mass from 86 to 344 kDa, providing exceptional versatility for diverse protein samples.
Mass photometry operates by measuring the interference between the light scattered by individual biomolecules and the light reflected from the measurement surface. This interference correlates directly with the mass of the biomolecules, allowing researchers to accurately determine the sample’s mass. To ensure precise mass measurements, calibrants with known mass serve as references. MassFerence P1 offers a standardized, dependable reference to optimize the mass conversion process.
Refeyn’s MassFerence P1 is designed for ease of use, providing a convenient solution for protein calibration. It is ready-to-use straight out of the box, requiring only a simple dilution. The use of Massference P1 enables faster workflows for data analysis in DiscoverMP software. The standard is compatible with a wide array of standard buffers, ensuring adaptability across various experimental conditions. Each box of MassFerence P1 includes three 50 µL aliquots, offering ample material for multiple uses. This ensures that researchers can consistently replicate their experiments across different laboratories and instruments, significantly enhancing the reliability and reproducibility of their data. Whether engaged in academic research, drug development, or protein characterization, MassFerence P1 stands as an invaluable tool for researchers employing mass photometry.
Refer to Page Number 3 in the Consumable User Guide on how to conduct calibration measurements
| Brand | Refeyn |
|---|---|
| Pack Size | 1 |
| Contents | 3 x 50 µL aliquots |
| Mass Range | 90 to 1000 kDa |
| Number of Experiments Conducted | Each vial is sufficient for 10 days of calibrations (assuming two calibrations a day) |
| Reusable | No |
| Instrument Compatability | OneMP, TwoMP, TwoMP Auto |
| Sample Compatibility | Any antibody, protein or protein complex between 90kDa and 1MDa |
| Volume in Tube | 50 µL aliquots |
| Sample Volume for Use | Aliquot 10 x 5µL upon first thaw for best results |
| Storage Instructions | Store at -20 ºC, stable for 6 months Aliquot 10 x 5 µL upon first thaw for best results |
| Shipment Conditions | Dry ice |
| Shelf Life | 6 months |
| Shelf Life After Opening | See Instructions on Product Data Sheet |
MassFerence P1 was engineered to deliver maximal precision for proteins with molecular weights ranging from 90 kDa to 1000 kDa, maintaining mass accuracy of less than 5%. One of its standout features is an automatic calibration capability integrated into our analysis software, which enhances consistency across different experiments, instruments, and laboratories. This ensures that your calibration processes are optimized for mass photometry.
MassFerence P1 is the recommended calibrant for OneMP and TwoMP, designed and validated to deliver results within specifications (<5% mass accuracy) for proteins ranging from 90 to 1000 kDa. The MassFerence P1 calibrant is packaged in a box with three 50 μL vials. Refeyn advises aliquoting the different tubes for long-term storage. For detailed usage instructions, refer to the Product Data Sheet for MassFerence P1 (MFP1 PDS).
No, the working solution (1:500 dilution from stock) is stable at room temperature for 24 hours in PBS. Outside of this condition, calibrants are not stable enough to provide the different oligomeric states required for the calibration.
No, Refeyn recommends 5 µL aliquots upon the first thaw of each vial to limit freeze-thaw cycles and ensure the best performance.
In the low-mass range, below 90kDa, Refeyn recommends using a protein commercially available with a molecular weight similar to the protein of interest. In case the particle molecular mass range is under and above 90kDa, we recommend performing two-step calibrations with MFP1 and a low-molecular-weight protein. More information can be found in our user manuals.
MassFerence P1 works with various standard buffers, but the oligomeric distribution might change based on the buffer chosen. This variation could lead to differences in peak counts but will not influence the calibration’s accuracy. For optimal calibration, select three or more peaks with >100 counts each, or use the automatic calibration software feature by selecting ‘MassFerence P1’ in AcquireMP when saving the acquisition.
