$220.00
MassFerence P2 is a calibration standard designed for mass photometry measurements of AAV-containing samples, ensuring precision and uniformity across experiments. Specifically designed for use with SamuxMP and SamuxMP Auto mass photometers, it facilitates accurate mass determination within a broad range of 670 kDa to 5 MDa.
Mass photometry is an advanced analytical technique. It measures the interference between light scattered by individual biomolecules in a sample and the light reflected by the measurement surface. The resulting signal, known as contrast, directly correlates with mass, allowing for precise mass calculations. To ensure accuracy, molecules of known mass, called calibrants, serve as reference points. MassFerence P2 provides a single standard protein species of 3640 kDa, enabling straightforward and reliable mass conversion. Given that the contrast-to-mass relationship remains linear within the relevant range, a single reference point ensures accurate calibration. MassFerence P2 is supplied in a dry form, with each box containing three vials, sufficient for 10 calibrations per vial once reconstituted. It is easy to prepare, requiring only simple dilution steps before use. The use of Massference P2 enables faster workflows for data analysis in DiscoverMP software.
To maintain product integrity, MassFerence P2 should be stored at 4°C in its dried form, where it remains stable for up to 9 months. Once reconstituted, the solution remains stable for 5 days at 4°C. Adhering to proper storage and handling guidelines ensures consistent performance and accurate mass measurements.
By providing a standardized reference, MassFerence P2 enhances reproducibility across different instruments and laboratories. It streamlines calibration workflows, making it an essential tool for gene therapy research, biopharmaceutical analysis, and biomolecular studies. Whether used in routine measurements or high-precision studies, MassFerence P2 supports reliable mass photometry calibration with minimal effort.
Refer to page number 4 in the linked consumable guide for instructions on how to conduct calibration measurements.
| Pack Size | 1 |
|---|---|
| Contents | 3 x vials in dry form |
| Mass Range | 670 to 5.000 kDa |
| Number of Experiments Conducted | Each vial is sufficient for 10 (manual) or 20 (auto) calibrations |
| Reusable | No |
| Sample Compatibility | AAV samples only, between 670kDa and 5 MDa |
| Instrument Compatibility | SamuxMP, SamuxMP Auto |
| Volume in Tube | Dry form |
| Sample Volume for Use | To resuspend in 120µL buffer |
| Storage Instructions | Dried calibrant stable for 9 months at 4ºC Solution stable for 5 days at 4ºC |
| Shipment Conditions | Room temperature |
| Shelf Life | 9 months |
| Shelf Life After Opening | See Instructions on Product Data Sheet |
MassFerence P2 is a reliable calibration standard for mass photometry measurements, particularly for AAV-containing samples ranging from 670 kDa to 5 MDa. Its advantages include easy use with simple resuspension, a single standard protein for accurate mass conversion, and support for consistent calibration with SamuxMP and SamuxMP Auto mass photometers. It enhances reproducibility, streamlines workflows, and is suitable for various gene therapy and biopharmaceutical research applications.
MassFerence P2 needs to be resuspended in 120 µL of buffer. In solution, MassFerence P2 remains stable for 5 days at 4°C. The instructions on how to use MassFerence P2 are indicated in the Product Data Sheet.
MassFerence P2 has been tested in various buffers commonly used in AAV purification processes, where the calibrant consistently provided accurate results. However, due to its nature, we cannot exclude the possibility that MassFerence P2 might be unstable in some buffers. As such, detergents should be avoided, as they significantly impact the product.
No, this will impact the product’s stability. MassFerence P2 remains stable 5 days after resuspension at 4°C due to its nature. Other storage conditions might affect the calibrant quality and reduce the calibration accuracy.
No, Refeyn does not recommend using MassFerence P2 on a TwoMP system. The nature of the MassFerence P2 calibrant makes it more visible in the low mass range, which can bias the results when using the TwoMP system, and the performance would not be guaranteed. Therefore, we do not recommend it for our TwoMP system.
MassFerence P2 can be reliably used as a one-point calibrant. Testing at Refeyn has shown no significant difference between one-point, two-point, or three-point calibrations. All three methods demonstrated a linear contrast-mass relationship and produced highly comparable results. More information and details can be found in our Tech Note, “Accurate mass calibration with MassFerence P2” (MFP2 Tech Note)
